ZFIN Image: Lobo et al., 2012, Fig. S2

Image

Figure Caption

Fig. S2 Blood phenotype of Bcdo2-MO1 morphants raised in the absence of PTU. (A) 3 dpf Bcdo2 morphant and control embryos (n=40 each) were stained for hemoglobin with o-Dianisidine as outlined in the Materials and methods. (B) Post Bcdo2-MO1 injection, 2-4 dpf larvae (n=50) were harvested and processed for total RNA as outlined in the Materials and methods. β-Globin (hbbe3) mRNA expression in Bcdo2 morphants was assessed using quantitative real-time RT-PCR, and compared with those of controls, harvested at the indicated time points. All larvae were raised in the absence of PTU. (C) Photographs of blood smears from 77 hpf control and Bcdo2-MO1 morphant fish raised in the absence of PTU.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development