ZFIN Image: Lobo et al., 2012, Fig. S1

Image

Figure Caption

Fig. S1 Characterization of Bcdo2-morphants generated with Bcdo2-MO2. (A) Scheme of the region of the Bcdo2 gene to which Bcdo2-MO2 was directed. The wild-type splice variant of the Bcdo2 mRNA is indicated by dotted lanes. The positions of the primers used for RT-PCR analysis are indicated in the figure. E, exon; I, intron. (B) Scheme of Bcdo2 mRNA with un-spliced intron 2. (C) Agarose gel electrophoresis of RT-PCR products for Bcdo2 generated from total RNA preparation of 3 dpf wild-type and four individual Bcdo2-MO2-treated larvae. (D) Representative lateral views of o-Dianisidine-stained 3 dpf wild-type and morphant larvae treated with different amounts of Bcdo2-MO2.

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Phenotype details

Acknowledgments

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