Fig. S9
LiCl treatment caused AF/MFF shrinkage. (A,B) Time-lapse observation of morphological change caused by LiCl treatment in the pectoral fin bud (A, 70 hpf) and the median fin fold (B, 42 hpf) using the hspGFFDMC131A;UAS:GFP fish line. (A) The fin bud (arrow) began to shrink, eventually reaching half of its original size (n=35/40). (B) The MFF was gradually influenced (magenta brackets), whereas LiCl treatment did not affect somite size (white brackets). (C,D) Cytoskeleton organization in the dorsal MFF (magenta bracket). The same effect was observed (control MFF in C; LiCl-treated MFF in D). Scale bars: 100 μm in A,B; 20 μm in C,D. Head is to the left and dorsal is to the top.