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Fig. S6
SU5402 treatment affects AF outgrowth. Inhibition of signaling through FGF receptors was performed with SU5402 (CalBiochem) as described previously (Abe et al., 2007). Embryos were incubated in 20 µM SU5402 (prepared from 4 mM stock solution in DMSO) containing fish water. Control embryos were incubated with the corresponding amount of DMSO. (A-D2) SU5402 inhibits both elongation of the AF and outgrowth of the endoskeletal region. hspGFFDMC131A;UAS:GFP line fish were treated with DMSO (A,B) or 20 μM SU5402 (C,D) for 24 hours. Cell shape was visualized by staining with F-actin (magenta) (A,C). (E) PD length of endoskeletal region (left) and the AF region (right) at 70 hpf after treatment with DMSO (n=12, white bars) or 20 μM SU5402 (n=11, red bars). Measurements are shown in supplementary material Table S3. Data were analyzed by Student’s t-test (**P<0.001). Error bars indicate s.d. Scale bars: 100 μm in A-D2.