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Fig. 2 Slow heart rate in par1 morphants.
(A) Heart rates were determined at 48 hpf and significance was described from an ANOVA-test statistic. The heart rate of par1 morphants was significantly lower than controls. *** p<0.001. n values were indicated. (B) Classification of heart rate. Class 1: heart shows no heart rate; class 2: heart presents lower heart rate <90 bpm; class 3: heart rate seems normal >90 bpm. (C) Ventricular shortening fraction (D) The heart is labeled with anti-myosin heavy chain antibody in 48 hpf embryos. Ventral view with the head on the top. a, atrium; v, ventricle, L; embryo left.
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