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Fig. 5 Zebrafish Scube2 binds SHH when co-expressed in COS-1 cells. Binding of Scube2-Myc to SHH-GFP occurs only in the membrane fraction. COS-1 cells were transiently transfected with Scube2-Myc and Shh-GFP. As a control, an empty Myc vector was co-transfected with SHH-GFP. Immunoprecipitation (IP) was performed on lysates and media fractions using anti-Myc antibody. Western blotting (WB) of immunoprecipitated proteins was performed using anti-GFP (upper panel) or anti-Myc (lower panel) antibodies.
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Reprinted from Developmental Biology, 368(2), Johnson, J.L., Hall, T.E., Dyson, J.M., Sonntag, C., Ayers, K., Berger, S., Gautier, P., Mitchell, C., Hollway, G.E., and Currie, P.D., Scube activity is necessary for Hedgehog signal transduction in vivo, 193-202, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.