ZFIN Image: Lee et al., 2012, Fig. 5

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Figure Caption

Fig. 5 Analysis of transient expression of GFP in response to oestrogens in medaka embryos. The plasmid pERE-Gal4ff containing the Tol2 transposase mRNA and the plasmid pUAS-GFP were injected into 1–2 cell stage medaka embryos and the embryos were exposed to 17α-ethynyloestradiol (100 ng EE₂/L) via the water. No GFP expression was detected in controls. The injected medaka embryos exposed to EE₂ showed strong GFP expression in the skin in the anterior region and in the body trunk (B). At 11 dpf, fish exposed to EE₂ showed GFP expression in the somite muscle, heart, liver and gall bladder (D, F, H and J).

Acknowledgments

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