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Fig. 3
Activation of Akt/PI3K pathways and nitric oxide (NO) production in X-13-treated HUVECs. Representative blots show that X-13 increased Akt and eNOS phosphorylation (A,B) while PI3K inhibitor LY294002 attenuated X-13-induced Akt and eNOS phosphorylation (C). Representative image shows that X-13 induced NO release while PI3K inhibitor LY294002, NOS inhibitor LAME, but not erk1/2 inhibitor PD98059 reduced X-13-induced NO release (D). The bar charts show quantitative data. Data are expressed as the mean ± SD. Results were obtained from three independent experiments (* X-13 vs. vehicle control group, p < 0.05; # LY294002 plus X-13 vs. X-13, p < 0.05; ☆ L-NAME plus X-13 vs. X-13, p < 0.05).