Fig. S2
Expression of bmp2a, bmp2b and ihha are unchanged in the gut of miR145 overexpressing embryos
Lateral view of whole mount in situ hybridization shows that bmp2a, bmp2b and ihha remain at similar expression levels after miR145 is overexpressed in the gut of 96 hpf embryos. Sections to the right of the whole mount embryo represent longitudinal sections of the whole mount embryos. Sections show the expression of bmp2a and bmp2b is only restricted to smooth muscle cell (arrowhead) and ihha is expressed in the epithelial cell (arrow). The magnification for AF is shown by the scale bar in F and A′F′ is shown by the scale bar in F′.
Reprinted from Developmental Biology, 367(2), Zeng, L., and Childs, S.J., The smooth muscle microRNA miR-145 regulates gut epithelial development via a paracrine mechanism, 178-186, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.