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Fig. S5 ihha regulates ventral outgrowth of the Op. Quantitation of dorsoventral (D/V) and anteroposterior (A/P) Op outgrowth during later larval development. (A) Example of how measurements were taken for overall A/P and D/V lengths on a 10 dpf wild-type Op stained with Alizarin Red. D/V length is a straight vertical line adjoining the dorsal tip of the joint (j apex) to the ventral-most apex on the anterior edge (v apex). A/P length is a straight horizontal line connecting the j apex to a distance equivalent to the posterior dorsal apex (p apex). (B) ihha mutants consistently show a significant decrease in D/V Op length compared with wild type, with the overall difference increasing with time. (C) A/P growth is not significantly different at any of the four time points. (D) ihha mutant A/P growth rate is relatively equivalent to the wild-type growth rate, but the D/V growth rate becomes increasingly disparate from wild type during development. Data are presented as mean±s.d., ***P<0.001, ne7 per genotype at each time point.