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Fig. 3
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- ZDB-IMAGE-120718-8
- Publication
- Cibrián Uhalte et al., 2012 - In vivo conditions to identify prkci phosphorylation targets using the analog-sensitive kinase method in zebrafish
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- Figures for Cibrián Uhalte et al., 2012
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Figure Caption
Fig. 3
Mutant PrkciI316A uses bulky N6-benzyl ATPγS.
Kinase reaction with Myelin Basic Protein (MBP) and ATPγS or N6-benzyl ATPγS (6-bn- ATPγS), followed by PNBM alkylation. In comparison, only mutant PrkciI316A efficiently utilizes N6-benzyl ATPγS to thiophosphorylate MBP. Labeled MBP is detected by Western blot analysis with rabbit monoclonal anti-thiophosphoester antibody (α-thioP).
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