Fig. 8 Over-expression of bmp2b specifically localized in the CHT area at 79 hpf upon ginger or 10-G exposure in normal and in anemic zebrafish embryos.
Whole-mount in situ hybridization of bmp2b. (A–C, left) Normal non-anemic control embryos or embryos treated with ginger/10-G. (D–F, right) Anemic control embryos or anemic embryos treated with ginger/10-G. Anemic groups were treated with 0.5 μM PHZ from 33 to 48 hpf. Embryos express bmp2b in the CHT region (arrows) following exposure to ginger (B, E) or 10-G (C, F). (G) A table shows the percentage of embryos with bmp2b expression in the CHT area at 79 hpf. Scale bars = 420 μm.