Fig. 2 Ginger/10-G treatment increases hematopoietic progenitor markers expression.
Zebrafish embryos were treated with ginger or 10-G from 9 to 21 hpf. (A) Tg(gata1:dsRed) for erythrocyte and Tg(flk1:GFP) for blood vessels, double-fluorescent overlay pictures of embryos at 22 hpf after exposure to ginger or 10-G. Hypertrophy of the PBI vascular plexus in Tg(flk1:GFP) after ginger or 10-G treatment, with Tg(gata1:dsRed) red fluorescent erythrocytes accumulated inside the honeycomb-like vasculature (arrows). Scale bars = 500 μm. Whole-mount in situ hybridization of c-myb (B) and scl (C) in zebrafish embryos at 22 hpf. Both hematopoietic progenitor markers were up-regulated in primitive hematopoietic tissues (ICM+PBI) upon ginger (arrow head) or 10-G (arrow) exposure. Scale bar = 350 μm.