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Fig. S2 Functional characterization of the LCR in transiently injected embryos. (A) Summary of the transgenic constructs injected including the enhancer element, promoter and vector backbone. All vectors listed contain a GFP reporter open reading frame. Bright field, GFP and composite images of representative Gata1Peak-LCR-MinPro-GFP (B) and DNaseIPeak-LCR-MinPro-GFP (C) embryos. 1The # Observed refers to all embryos observed from the injected clutch. 2The # Observed refers to the number of productively injected embryos as determined by expression by an mCherry reporter plasmid.
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Reprinted from Developmental Biology, 366(2), Ganis, J.J., Hsia, N., Trompouki, E., de Jong, J.L., Dibiase, A., Lambert, J.S., Jia, Z., Sabo, P.J., Weaver, M., Sandstrom, R., Stamatoyannopoulos, J.A., Zhou, Y., and Zon, L.I., Zebrafish globin switching occurs in two developmental stages and is controlled by the LCR, 185-194, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.