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Figure Caption
Fig. S1 ring1b mRNA and protein are maternally deposited. (A,B) Whole-mount in situ hybridization with a riboprobe against ring1b shows expression at the eight-cell stage, no staining is detected with the sense probe. (C) Western blot analysis of Ring1b protein in wild-type embryos at 24 hpf as control and 2.5 hpf shows maternal Ring1b protein. (D) Ring1b protein is detected in lysates of ring1b mutant embryos at 15 somites but is not or is hardly detectable at 24 hpf (D). Histone H3 is used as a loading control.
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