ZFIN Image: Stevenson et al., 2012, Fig. 1

Image

Figure Caption

Fig. 1

Hypoxia disrupts axon pathfinding in the developing CNS.

Confocal images are maximum intensity projections of whole-mount embryos, ventral views, rostral top, scale bars 50 μm. (A, B) 48 hpf embryos, acetylated tubulin immunohistochemistry. (B) Following 1% hypoxia (1% pO₂) from 24–36 hpf, anterior commissure and post-optic commissural tracts are disrupted (arrows). (C–I) Tg(foxP2-enhancerA.2:egfp-caax) embryos (abbreviated foxP2-A.2:caax), GFP immunohistochemistry. (C) Normoxic embryo at 72 hpf; TCPT commissure (TCPTc) (arrow). (D) Embryo following hypoxia from 24–36 hpf; TCPTc is absent (arrow). (E) Graph showing percent Tg(foxP2-A.2:caax) embryos with wild-type TCPTc following hypoxia exposure at different ages. Error bars standard error of the proportion; ** p<0.0001. (F) C/L ratio determination. Confocal image of 72 hpf embryos illustrating determination of ratio of commissure intensity to intensity of longitudinal axons. (G) Quantification of TCPTc errors in Tg(foxP2-A.2:caax) embryos following hypoxia exposure at different ages. C/L (commissure:longitudinal) intensity ratio quantification along y-axis. *p<0.05; ** p<0.0001. ^mark indicates significant mortality at this stage. Errors bars standard error of the mean. (H) Normoxic embryo at 96 hpf; TCPT is intact (arrow). (I) Embryo at 96 hpf following 1% hypoxia from 24–36 hpf; TCPTc is absent (arrow).

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.