ZFIN Image: Stevenson et al., 2012, Fig. 5

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Figure Caption

Fig. 5

ephrinB2a mediates the hypoxia-induced TCPT pathfinding errors.

(A–B) ephrinB2a is expressed in TCPT neurons. Confocal maximum intensity projections of whole-mount embryos, double immunohistochemistry for GFP and EfnB2a, ventral views, rostral top, scale bar 50 μm. (A–A3) TCPT neurons express ephrinB2a as they begin to extend axons. (B–B3) TCPT commissural axons express ephrinB2a as they cross the midline. (C–D) Hypoxia leads to increased expression of EfnB2a. Maximum intensity projections of 36 hpf embryos, ventral views, rostral to the top, EfnB2a immunohistochemistry. (E) Quantification of EfnB2a levels, normalized to normoxia. *p<0.05. Error bars SEM. (F) Knockdown of EfnB2a expression with morpholino rescues TCPTc pathfinding. Tg(foxP2-A.2:caax) embryo at 72 hpf; confocal maximum intensity projection, ventral view, rostral top, scale bar 50 μm. (G) C/L intensity ratio quantifications show ephrin morpholino rescue of hypoxia pathfinding errors. *p<0.01; ** p<0.001. Error bars SEM. (H) C/L intensity ratios show UAS:ephrinΔc rescues hypoxia pathfinding errors. ** p<0.001. Error bars SEM.

Acknowledgments

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