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Fig. 5
In vivo enhancer-blocking assay of HCNRs mapping the human IRXB locus.
30 hpf zebrafish injected with the insulator-vector assay lacking any HCNR (panel A) and C91 (panel B). With no insulator activity, Z48 enhancer interacts with the cardiac actin promoter promoting EGFP expression to the midbrain (C). Whenever an insulator is placed between the enhancer and the promoter, midbrain expression is reduced when compared to the somites expression, which remain unaffected. Adapted from Bessa et al, 2009. E) Wisker-plot representation of the midbrain/somite ratios from different regions tested.
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