Fig. S3

Fig. S3

Osteoblasts and adjacent inter-ray cells are labeled in Class 3 mosaic fins. (A) Whole mount image of a Class 3 mosaic Tg(dusp6:Cre-ERT2, EAB:EGFP-FlEx-mCherry) fin demonstrating lepidotrichia-localized osteoblasts (white arrows) and adjacent non-osteoblast cells (yellow arrows). (B–I) Transverse sections from the same non-regenerating fin imaged for mCherry expression (B, F, red) and stained with either zn-3 or zns-5 antibodies (C, G, green) showing mCherry⁺/(zn-3 or zns-5)⁺ osteoblasts and inter-ray, non-osteoblast mCherry⁺/(zn-3 or zns-5) cells. Nuclei are stained with Hoechst (D, H, blue), and the overlays are shown (E, I). White arrows indicate mCherry⁺/(zn-3 or zns-5)⁺ osteoblasts and yellow arrows indicate mCherry single positive cells.