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Fig. 3
Tether cilia are immotile even in the absence of otoliths. (A) DIC image of a <24 hpf kei mutant showing anterior tether cilia (inset, black asterisks) without any bound otolith, although otolith precursor particles are present. Scale bar: 40 μm. (B) Confocal stack through the OV of a 27S kei mutant embryo. Cilia appear normal (arrowheads indicate tether cilia). Scale bar: 20 μm. (C) Time-to-colour merge of six frames of a high-speed movie of a 24S kei mutant embryo. Inset: two immotile kinocilia (marked by black asterisks) and one motile cilium (marked by white asterisk). Scale bar: 20 μm. See supplementary material Movie 9. (D) Time-to-colour merge of six frames of a high-speed movie of a 24.5 hpf kei mutant embryo; kinocilia (black asterisks) are immotile. White asterisks mark motile cilia. Scale bar: 6 μm. See supplementary material Movie 10.