|
Fig. S7
Validation of par-3 morpholino antisense oligonucleotides and expression of her4.1 and dla in mib/par-3 double deficient embryos, related to Figure 8. Injection of 0.35 pmoles par-3 morpholino results in mild defect in brain morphology at 26 hpf (B), while 0.5 pmoles par-3 morpholino results in severe defect in brain morphology at 26 hpf (C), compared with injection of control morpholino (A). At 37 hpf, the morphologic defects of both 0.35 pmoles (E) and 0.5 pmoles par-3 morpholino (F) recover to certain extent, compared with injection of control morpholino (D). (A) and (D), (B) and (E), (C) and (F) represent the same embryo at different developmental stages respectively. Immunostaining of aPKC shows that injection of both 0.35 pmoles (H) and 0.5 pmoles par-3 morpholino (I) can lead to loss of apico-basal cell polarity, as compared with injection of control morpholino (G). (J and K) FISH of her4.1 in control morpholino-injected embryo of mib +/- (J) or mib -/- (K). (L and M) FISH of her4.1 in par-3 morpholino-injected embryo of mib +/- (L) or mib -/- (M). (N and O) FISH of dla in control morpholino-injected embryo of mib +/- (N) or mib -/- (O). (P and Q) FISH of dla in par-3 morpholino-injected embryo of mib +/- (P) or mib -/- (Q).