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Fig. S5
Validation of the dla morpholino antisense oligonucleotides, related to Figure 6. (A) Effect of the dla morpholino on general morphology of 36 hpf embryos. Control morpholino (upper) and dla morpholino (lower) are injected at the 1-cell stage. (B) Effect of the dla morpholino on neurogenesis in 24 hpf embryos. Immunohistochemistry of Hu indicates that Hu positive cells are significantly increased in dla morpholino injected embryos (lower) compared with control morpholino-injected embryos (upper). tc: telencephalon, dc: diencephalon, mb: midbrain, hb: hindbrain.
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