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Fig. S11
Western blot analyses of human GLE1 expression in zebrafish embryos. gle1 morphants injected with buffer alone (-), wild-type human GLE1b mRNA (+WT), or human GLE1b_FinMajor mutant mRNA (+FinMajor) were lysed at the bud stage and subjected to western blot analyses using the anti-human GLE1 antibody (which does not recognize the zebrafish Gle1 protein). Anti-actin staining was also performed as a loading control. Note that both the wild-type and the FinMajor form of human GLE1 proteins were expressed in zebrafish embryos.
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