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Fig. S3
Anti-Islet1/2 staining of zebrafish at 48 hpf. An example of anti-Islet1/2 staining to reveal both the Rohon-Beard sensory neurons (which are the large cells close to the dorsal edge) and motoneurons (at the ventral edge of the spinal cord). Note that the gle1-/- mutant has fewer motoneurons than the wild type. See text and Table 1 for quantitative analysis. Images are lateral views of the trunk spinal cord with dorsal to top, anterior to left. Scale bar: 75 μm.
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