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Fig. 2
Application of 10-5M diethylaminobenzaldehyde (DEAB) to zebrafish embryos. A:tbx5 positive fin precursors are absent at 24 hpf in embryos treated from shield, 75% epiboly and tail bud stage but are detectable if treatments are initiated at the 6-somite (s) stage; dorsal view; anterior is to the left. B: DEAB treatments throughout somitogenesis lead to loss of tbx5 expression and fin bud development at 32 hours postfertilization (hpf). Later treatments lead to reduction of tbx5 expression and fin bud size at 32 hpf; lateral view, anterior is to the left. C: DEAB treatments during early to midsomitogenesis reduce tbx5 expression and fin bud size at 32 hpf; lateral view, anterior is to the left. D: Schematics of inhibitor treatments and results.