ZFIN Image: Kirchmaier et al., 2012, Fig. S7

Image

Figure Caption

Fig. S7 Craniofacial bone and cartilage formation is not affected in popdc2 morphants. (A,B,C,D) Left lateral and (A2,B2,C2,D2) ventral views of (A,A′,B,B′) MO-CTR and (C,C′,D,D′) MO1-popdc2 injected embryos, which were stained for bone (alizarin red) and cartilage (alcian blue) at (A,A′,C,C′) 4 dpf and (B,B′,D,D′) 5 dpf respectively. At 4 dpf, popdc2 morphants (n = 12/13) display normal pattern of cartilage and bone formation as the control (n = 17). At 5 dpf, most of the morphants (32/39) showed a normal distribution of bone and cartilage elements of MO1-popdc2 when compared to the controls (n = 24).

Acknowledgments

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Reprinted from Developmental Biology, 363(2), Kirchmaier, B.C., Poon, K.L., Schwerte, T., Huisken, J., Winkler, C., Jungblut, B., Stainier, D.Y., and Brand, T., The Popeye domain containing 2 (popdc2) gene in zebrafish is required for heart and skeletal muscle development, 438-450, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.