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Fig. 4 neo1 expression is reduced in Shh/Gli pathway mutants and increased on Shh/Gli pharmacological activation. neo1 is expressed broadly in the developing CNS (A). Expression in 48 hpf embryo can be detected in the otic vesicle, in the notochord (B, close-up D, black bracket) and in the eye (E, black arrowhead), probably in the retinal ganglion cell layer (gcl). (C) Cartoon of a 48 hpf zebrafish eye. Blue line shows neo1 expression in the gcl. At 48 hpf neo1 expression is diminished in gli1 (dtr) and smu (smo) mutants, specifically in the notochord (F, H, bracket) and the gcl in the optic placode (G, I; black arrowhead) in comparison to WT (D, E). (J, K) neo1 mRNA is increased in notochord. Note that (J) and (K) controls were developed at different times in comparison to (D) in order to match to their corresponding pur treatment (K). Anterior is towards left, lateral views are showed except in (E, G, I, K) with dorsal views anterior up. Scale bars; 50 μm except in A (100 μm). WT; wild type. gcl; retinal-ganglion cell layer. DMSO is the vehicle for pur.