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Fig. 2 Knockdown of rfx2 gene function results in altered body shape, and LR patterning defects in heart and gut. Rfx2 morphants (B) exhibited a strongly ventrally curved trunk and tail compared to un-injected wild-type embryos (A). The direction of heart and gut looping were often reversed in rfx2 morphants (C–H). In a wild-type embryo, the atrium of the heart loops toward the left side of the embryo (C) and the liver and intestinal bulb are located on the left side of the embryo (E) while in rfx2 morphants the position of these organs was often reversed (D, F). B, D, F - rfx2 AUGMO. Histogram depicting the percentage of heart and gut reversals in wild-type and rfx2 morphant embryos (G). The incidence of gut reversals in rfx2 AUGMO embryos was partially rescued by injection of rfx2 RNA. (H) Histogram depicting the percentage of heart reversals in wild-type and rfx2 AUGMO embryos injected with rfx2 RNA.
Reprinted from Developmental Biology, 363(1), Bisgrove, B.W., Makova, S., Yost, H.J., and Brueckner, M., RFX2 is essential in the ciliated organ of asymmetry and an RFX2 transgene identifies a population of ciliated cells sufficient for fluid flow, 166-178, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.