Fig. 2

Fig. 2 The effects of compounds on DASPEI and FM1-43FX staining in the pLL. Ototoxin treatment reduced DASPEI and FM1-43FX staining in the hair cells of the pLL compared to control larvae. Other staining, including the nasal epithelium and ear, was not affected in treated larvae (Rows A–F). Treatment with negative control compounds did not reduce the brightness of DASPEI or FM1-43FX labelling in the pLL compared to control larvae (Rows G–I). The images shown are representative of all fish tested (12 larvae per treatment group). Scale bar = 800 μm. (Ji) Schematic showing typical pattern of neuromasts in a 5 dpf zebrafish larva. Smaller neuromasts from the second wave of deposition are indicated (II-1, II-2), but were not scored for the purpose of the DASPEI assay. The remaining 9 neuromasts of the pLL (P1-9) were scored on both sides. (Jii-iii) Exemplar images of neuromast staining with DASPEI. Image (Jii) is representative of a healthy neuromast given a score of 2 in the fluorescence assay. Image (Jiii) is representative of a damaged neuromast, given a score of 1. Scale bar = 20 μm.