Fig. 3 Analysis of myogenic markers expression and muscle pioneers in ccdc80-l1 morphant embryos.
(A–D) The myogenic markers myod (A, B) and myog (C, D) were correctly expressed both in control and morphants embryos at 10 s stage (A, B) and 24 hpf (C, D), respectively. (E, F) The MF20 antibody staining showed that both slow and fast twitch fibers were correctly formed and distributed in control and in knocked-down embryos at 24 hpf. (G, H) At the same developmental stage, muscle pioneers resulted unaffected after ccdc80-l1 loss-of-function, as shown by the labeling with 4D9 antibody (anti-engrailed) (arrows). (A, B) Dorsal views, anterior is left; (C–H) lateral views of the tails, dorsal is up and anterior is left.