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Fig. S4 olig2 is not expressed in r4 and olig2 loss of function has no effect on hoxb1a or prickle1b expression. A: Wild-type expression at 22 hpf of olig2 in r5 and r6 in blue and egr2b in r3 and r5 in red. B: qPCR of hoxb1a expression in wild-type, control MO-injected, and olig2 MO-injected embryos at 24 and 33 hpf. C: qPCR of prickel1b expression in wild type, control MO-injected, and olig2 MO-injected embryos at 24 hpf. Error bars represent standard deviation. P value calculated by Student′s t-test is listed above each bar and compared to wild-type embryos.
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