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Fig. S4 The ablated primitive myelopoiesis in gata4/5/6-depleted embryos can be rescued by treating with 10 μM DEAB starting before 11 hpf. All embryos were positioned anterior left and lateral front. Embryos were microinjected with gata4-MO and gata6-MO at 1–2-cell stage (B–H, J–P) and then treated with vehicle DMSO (B, J) or 10 μM DEAB continuously starting from 1–2-cell stage (C, K), 3 hpf (D, L), 5 hpf (E, M), 7 hpf (F, N), 9 hpf (G, O), and 11 hpf (H, P), respectively. The embryos were then grown together with wild type control embryos (A, I) to 24 hpf for examining expressions of myeloid markers lcp1 (A–H) and mpx (I, P) respectively. The number shown in the lower left-hand corner of each panel is the number of embryos exhibiting the typical phenotype shown in the panel to the number of embryos totally observed. Treatment with DEAB before 11 hpf can well rescue the abolished primitive myelopoiesis in gata4/5/6-depleted embryos (C–G; K–O) whereas the treatment after 11 hpf hardly rescued the primitive myelopoiesis (H, P).