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Fig. S2 qRT-PCR analysis shows overexpression of scl and lmo2 into zebrafish embryos partially rescues the defective primitive myelopoiesis in the embryos treated with 250 nM RA during 10–11 hpf. Embryos were treated with vehicle DMSO (WT), microinjected with scl and lmo2 mRNA at 1–2 cell stage (scl/lmo2 mRNA), treated with 250 nM RA during 10 to 11 hpf (250 nM RA 10–11 hpf), or microinjected with scl and lmo2 mRNA at 1–2-cell stage and then treated with 250 nM RA during 10 to 11hpf (RA+scl/lmo2 mRNA), respectively. To exclude the myeloid cells derived from ICM, embryos that were removed tails and trunks at 24 hpf were used to detect relative expression levels of myeloid markers lcp1 and mpx by qRT-PCR. Overexpression of scl and lmo2 did not change expression levels of lcp1 and mpx in wild type embryos but significantly rescued the inhibited expressions of lcp1 and mpx in the embryos treated with 250 nM during 10–11 hpf. **: P<0.01.