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Fig. 8 aldh1a2 is one of downstream target genes of gata4/5/6 to affect zebrafish primitive myelopoiesis.
Embryos are positioned animal pole top and ventral front (A–I), anterior top and dorsal front (J–O), and anterior left and lateral front (R–W). Embryos were microinjected with control MO (A, D, G, J, M, R, U), gata5-MO plus gata6-MO (B, E, H, K, N), gata4 mRNA plus gata6 mRNA (C, F, I, L, O), gata5-MO and gata6-MO plus control MO (S, V), gata5-MO and gata6-MO plus aldh1a2 MO (T, W) at 1–2-cell stage, respectively. They were then examined for expressions of aldh1a2 at 5 hpf (A–C), 7 hpf (D–F), 9 hpf (G–I), 11 hpf (J–L) and 13 hpf (M–O), lcp1 (R–T) and mpx at 24 hpf (U–W) by whole mount in situ hybridization, respectively. qRT-PCR was performed to confirm the relative expression level changes of aldh1a2 in gata4/5/6 depleted embryos (P) or in gata4/6 overexpressed embryos (Q) at 5, 7, 9, 11, 13 hpf, and those of lcp1 and mpx at 24 hpf (X). The number shown in the lower left-hand corner of each panel (R–W) is the number of embryos exhibiting the typical phenotype shown in the panel to the number of embryos totally observed. *: P<0.05; **: P<0.01.