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Fig. 3 Rheotaxis depends on level of hair cell impairment or damage and is restored during regeneration.
A) Mechanotransduction, as shown by FM1?43 dye uptake, is blocked when tip-links are broken at ~30 min after EDTA treatment (acute) and is restored again at 4?5 hours post treatment (recovery) (flow = 0.16 cm/s). B) Rheotaxis is impaired acutely after EDTA treatment and restored to control levels after recovery (n = 5). C) Ablating the lateral line input by different neomycin doses leads to a dose dependent rheotaxis impairment (n = 6) (flow = 0.14 cm/s). D) Rheotaxis is restored during mechanosensory hair cell regeneration (n = 5) (flow = 0.16 cm/s). Statistics: C) Statistics: ANOVA with Tukey post-tests. B,D) Paired t-test. Error bars show standard deviation. * p = 0.01?0.05, ** p = 0.01?0.001, *** p<0.001.