Fig. S1
Cx43-mApple and Cx40.8-EGFP co-assemble in common gap junction channels. High resolution fluorescence microscopy was used to provide evidence for co-association of Cx43-mApple and Cx40.8-EGFP in common gap junction channels. Plane views are shown. Top: In HeLa cells co-transfected with Hs-Cx43-EGFP and Hs-Cx43-mApple, both green and red plaques completely overlap. Middle: In HeLa cells co-transfected with Hs-Cx26-GFP and Hs-Cx43-mApple, discrete domains of green and red plaques are observed. Bottom: In HeLa cells co-transfected with Dr-Cx40.8-EGFP and Dr-Cx43-mApple, both green and red plaques completely overlap.