Fig. 6
Untagged Cx43 and Cx40.8 constructs behave similarly as GFP tagged constructs in transiently transfected HeLa cells.
(A) Cx43 localizes to the plasma membrane. (B) Cx40.8 is retained intracellularly. (C) Cx43-bBCD is retained intracellularly. (D) Cx40.8-cd localizes to the plasma membrane. The Cx43 antibody [12] was used to detect untagged Cx43 and untagged Cx43-bBCD. The Cx40.8 antibody was used to detect untagged Cx40.8 and untagged Cx40.8-cd. Flared arrows identify gap junction plaques located at the plasma membrane; Plain arrows identify the plasma membrane in the absence of gap junction plaques; n, nucleus.