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Fig. S5
Knockdown efficacy of ponzr1 MOs. (A) Wild-type embryos show a very low level of baseline GFP autofluorescence. This GFP level was defined as 0% and used to standardize between the three independent experiments. (B) Embryos were injected with ponzr1 52 UTR-GFP capped mRNA. This GFP level was defined as 100% for each experiment to standardize between the three experiments. (C,D) ponzr1 MO1-injected embryos show a significant loss of fluorescence as compared to both ponzr1 52 UTR-GFP mRNA and mismatch MO at 1 ng (C) and 2 ng (D) doses. (E,F) ponzr1 MO2-injected embryos also show a significantly decreased fluorescence as compared with ponzr1 52 UTR-GFP mRNA and mismatch MO at both 2 ng (E) and 3 ng (F) doses. (G,H) Mismatch MO-injected embryos show an attenuated knockdown response, demonstrating only a 50% knockdown at both 2 ng (G) and 3 ng (H). (I) Percentage loss of GFP in MO-injected embryos. ponzr1 MO1 and MO2 are significantly different from both mismatch MO and ponzr1 52 UTR-GFP (***P<0.001).