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Fig. 7
Recovery of retinal tissue architecture and marker gene expression after photoreceptor ablation.
A, B) Hematoxylin-eosin staining of control retina after one month. dn-fgfr1 transgenic, regenerated retina has recovered a similar layered structure as the retina of wild-type control siblings. C, D) Expression of the double cone marker Zpr1 is recovered in the photoreceptor cells of dn-fgfr1 fish (arrowheads) after seven days of regeneration and displays a pattern comparable to control retinas. E, F) rho expression recovers in transgenic fish and is highly comparable to control fish (arrowheads). G, H) UV opsin (opn1sw1) expression shows the same pattern in control and in transgenic retinas (arrowheads). I, J) Expression of the downstream target gene etv5b, indicative of Fgf signaling activity, has recovered in dn-fgfr1 fish after seven days of regeneration and expression is indistinguishable from control fish (arrowheads). Scale bar = 20 μm.