ZFIN Image: Dohn et al., 2012, Fig. 2

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Figure Caption

Fig. 2

Wnt signaling is necessary for CM formation in the pregastrula embryo. (A–F) myl7, (G–L) vmhc and (M–R) amhc expression in representative HCSEs and embryos when Wnt signaling is decreased using the Tg(hsp70l:dkk1-GFP)^w32 line at the designated stages. (B,H,N) Wnt signaling is necessary prior to gastrulation to promote ventricular and atrial marker expressions. (C, I) Decreasing Wnt signaling at the initiation of gastrulation (shield stage) causes aberrant morphology of CMs expressing myl7 and vmhc. (O) Decreasing Wnt signaling at the initiation of gastrulation reduces the amount of cells expressing amhc. (D–F,J–L,P–R) The expression of CM differentiation markers is not affected when Wnt signaling is decreased from TB through 16 s. (S) Graph indicating the relative difference in area of cells expressing these markers. Asterisks indicate a statistical difference in the areas from HCSEs and GFP + embryos in Supplemental Table 1 according to Student′s t-test.

Acknowledgments

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Reprinted from Developmental Biology, 361(2), Dohn, T.E., and Waxman, J.S., Distinct phases of Wnt/β-catenin signaling direct cardiomyocyte formation in zebrafish, 364-76, Copyright (2012) with permission from Elsevier. Full text @ Dev. Biol.