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Fig. 7
Soluble THSD7A-mediated angiogenic effects are blocked with anti-sTHSD7A antibody.
A. The motility of HUVEC was evaluated by transwell migration assay. HUVEC were loaded in the upper insert. Control medium (V) or soluble THSD7A (T) in the presence (+) or absence (-) of anti-sTHSD7A antibody were added to the lower chamber. Bar represents 100 μm. B. Immunostaining of actin filaments (red) and nuclei (blue) in HUVEC treated with control medium (V) or soluble THSD7A (T) in the presence (+) or absence (-) of anti-sTHSD7A antibody. The quantitative data show the average number of filopodia per cell. C. Immunostaining of vinculin (green) and nuclei (blue) in HUVEC treated with control medium (V) or soluble THSD7A (T) in the presence (+) or absence (-) of anti-sTHSD7A antibody. The quantitative data show the average number of peripheral vinculin plaques per cell. Bar represents 10 μm. *P<0.05. **P<0.01. Each experiment was repeated at least three independent times.