Fig. S2

Fig. S2 Generation and validation of Cldn15lb peptide antibody. a, Sequence alignment between zebrafish Cldn10, Cldn15, Cldn15lb-a, Cldn15lb-b, and Cldn10l. [Cldn15lb-a and -b are the proteins encoded by cldn15lb_tv1 and cldn15lb_tv2, respectively.] Purple shading boxes indicate transmembrane regions. Red box highlights the epitope targeted by the peptide antibody. b, HEK293 cells were transiently transfected with constructs expressing Cldn10l, Cldn15, or Cldn15lb-a. Immunofluorescence with peptide antibody generated against Cldn15lb recognized Cldn15lb-a only. c, Cos7 cells were transiently transfected with untagged or GFP-tagged version of either transcript variant. The antiserum recognized cells that were transfected with either untagged transcript variant and it co-localized with the GFP-tagged version. Thus, the polyclonal antibody in the serum recognizes Cldn15lb but does not distinguish between the 2 isoforms.