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Fig. S5 grhl2a is involved in axial, but not MHB, patterning. (A,B) MO-mediated knockdown of grhl2a leads to defects reminiscent of those in mutants with failed convergence-extension mediated movements, characterised by a shorter, broader trunk relative to controls. (C-F) The expression of MHB markers (arrows) is not lost following MO-mediated knockdown of grhl2a (pax2a; C-D), compared with the loss seen after MO-mediated knockdown of grhl2b (pax2a; E,F). (G,H) Following MO-mediated downregulation of both grhl2a and grhl2b, severe CE-like defects are seen in axial patterning, formation of the notochord (highlighted by brightfield microscopy and in situ hybridisation expression of the specific notochord marker, ntl; inset), with only minor defects in the formation of the MHB constriction (bracket; H).