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Fig. 2
Segmentation of cell nuclei.
A) Original nuclei (scale bar 50 microns) taken from the gold standard data set, cell density 1150 cells/mm2. B–H) Nuclei with segmentation examples overlaid. Ellipses indicate segmentation errors. Lines indicate unresolved clusters of cells. B) Manually marked cell position. C) Cellomics segmentation. D) Seeded Growth. E) Global Threshold. F) Local Threshold. G) Scaling Index. H) CellProfiler. I) Cell detection accuracy measurements: Total cell count, false negatives and false positives comparing different segmentation methods to the gold standard.
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