ZFIN Image: Blum et al., 2012, Fig. S4

Image

Figure Caption

Fig. S4 Inhibition of RA signaling does not enhance cell death during blastema formation. Inhibition of RA signaling in hsp70:cyp26a1 fish does not enhance the number of TUNEL-positive cells in the fin stump (daily heat shocks, first heat shock 2 hours before amputation). (A) Longitudinal sections stained for TUNEL show few TUNEL-positive cells in the epidermis of wild-type and hsp70:cyp26a1 fins at 32 hpa. (B) Quantification of TUNEL-labeled cells within a defined area at 32 hpa (wild type, n=32 sections; hsp70:cyp26a1, n=25). Error bars, s.e.m. ns, not significant. Dashed lines indicate the amputation plane. hs, heat shock. Scale bar: 100 μm.

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