ZFIN Image: Carten et al., 2011, Fig. 5

Image

Figure Caption

Fig. 5 Egg yolk feeding enhances the uptake and metabolism of BODIPY fatty acid analogs by zebrafish larvae. Thin layer chromatography analysis of total larval lipids extracted following analog feeds in the absence (lanes 1–5) and presence (lanes 7–11) of egg yolk. Lanes 6 and 12 are no analog (NA) unfed and yolk fed controls, respectively, and exhibit faint naturally fluorescent lipid bands (NFB). A polar solvent system was used to resolve fluorescent phospholipids (bottom of plate), free analogs (middle), and neutral lipids (top of plate). Abbreviations for fluorescent lipid standards are as follows: BODIPY-Cholesteryl (B-CE-C₁₂), BODIPY-Triacylglyceride (B-TAG-C₁₂), BODIPY_493/503 (B), BODIPY-C₁₆ (B-C₁₆), and BODIPY-Phosphatidylcholine (B-PC-C₁₂, B-PC-C₅).

Acknowledgments

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Reprinted from Developmental Biology, 360(2), Carten, J.D., Bradford, M.K., and Farber, S., Visualizing digestive organ morphology and function using differential fatty acid metabolism in live zebrafish, 276-85, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.