Fig. 6

Fig. 6 Increased cell division of LsDh precursors in sec61al1^c163 mutants. Embryos were briefly pulsed with BrdU at 32 hpf and chased until 4 dpf, when they were labeled for Kctd12.1 (green) and BrdU (red). As neurons are post-mitotic, brightly labeled BrdU+ cells are those that underwent a final mitosis during the BrdU pulse (white arrows); see text for further explanation of the labeling strategy. (A–A′′′) Compared to WT controls, (B–B′′′) significantly more neurons are born in the RHb of sec61al1^c163 mutants at 32 hpf. This trend continues through 48 hpf (A′′′,B′′′), as again there are significantly more neurons in the RHb of sec61al1^c163 mutants (images not shown) (Andres et al., 1999). Images are single z-plane images from confocal z-stacks. Dorsal (A,B), mid (A′,B′), and ventral (A′′,B′′) sections are shown. Scale bar represents 30 μm. Significance determined by Student′s T-test (*p < 0.05; **p < 0.01); n = 41 siblings, n = 43 mutants.