Fig. 2

Fig. 2 Kctd12.1 expression is significantly expanded and Kctd12.2 expression significantly decreased throughout larval development in c163 mutants. (A, B, C) In wild-type larvae at 48 hpf, there are 2.1 times as many Kctd12.1-expressing neurons (green) in the left LsDh of the dorsal habenula as in the right. In the MsDh, there are 1.3 times as many Kctd12.2-expressing neurons (red) in the right side as the left. (A′, B′, C′) In c163 mutants, more Kctd12.1+ and fewer Kctd12.2+ neurons are found in the right habenula. (D–F′) At 72 hpf, a similar situation to 48 hpf is observed. Images are 3D reconstructions of 50 μm-thick confocal Z-stacks through the epithalamus. Scale bars represent 10 μm. Significance determined by Student′s T-test (*p < 0.05, **p < 0.01); n > 6. Insets in A–B′, D–E′ show ToPro3 labeling in a single confocal section of the Z-stack.