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Fig. 5 BMP signaling is required cell autonomously in NCPCs for their specification.
(A) Z-projection of confocal sections showing that wild-type donor cells transplanted into wild-type hosts readily express the neural crest marker Foxd3 at the 3-somite stage. (B,C,D) Single confocal section of the embryo in A. Foxd3 (B) and lineage tracer rhodamine dextran (C) are found in the same cells (D, arrowheads). (E) Z-projection of confocal sections showing that smad5 morphant donor cells within the neural crest region of a wild-type host do not express the neural crest marker Foxd3. (F, G, H, I, J, K) Single confocal sections of the cells indicated by arrows in E. Foxd3 (F, I) and lineage tracer (G, J) do not colocalize (H, K). (L, M, N) Single confocal section of a different host embryo containing smad5 morphant cells (Rho) within the neural crest region that do not express Foxd3. (O, P, Q) Z-projection of confocal sections of chimera in which donor cells were induced as ectopic neural crest. Foxd3 (O) and lineage tracer (P) colocalize (Q) in a patch of cells (arrowhead) located ventrally from the normal neural crest domain (asterisk).