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Fig. 9 Manipulating Gli activity in embryos with abrogated Hh signalling. (A-L) Lateral views of spinal cord expression in 24-hpf zebrafish embryos treated with cyclopamine (cyc) and injected with either gli2a, gli3 and p53 MOs (A,D,G,J), gli1 and p53 MOs (B,E,K) or gli3R RNA (C,F,I), a 24-hpf gli1 mutant (detour) embryo treated with cyclopamine (H) and a 6-somite (12-hpf) embryo treated with cyclopamine and injected with gli1 and p53 MOs (L). For L, 28/29 injected embryos had a complete lack of spinal cord olig2 expression; the remaining embryo had a very small amount of spinal cord staining. Scale bar: 50 μm in A-K; 250 μm in L.